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Coding
Part:BBa_K4395009:Design
Designed by: Yaxuan Wu Group: iGEM22_SYSU-CHINA (2022-09-29)
SpyTag-vvGT2-SpyCatcher
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 66
Illegal SpeI site found at 1014 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 66
Illegal SpeI site found at 1014 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 66
Illegal SpeI site found at 1014 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 66
Illegal SpeI site found at 1014 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The idea of fusing SpyTag and SpyCatcher on the N-terminus and C-terminus, respectively, on the enzyme vvGT2 enables the fusion protein to catalyze glucosyl transfer in higher temperature than one where the reaction normally happens.
Source
The SpyTag/SpyCatcher system is a derivative of CnaB2 domain of Streptococcus pyogenes vvGT2 comes from Vitis vinifera.